Association of Intelectin Gene Polymorphism with Type 2 Diabetes Risk in the North Indian Population

Singh Shivangi; Chaturvedi Shubhangi; Pooja; Modi Dinesh Raj  lowast; Singh Shefali; Saxena Madhukar; Katiyar Tridiv

doi:10.58739/jcbs/v15i1.122

Article

Association of Intelectin Gene Polymorphism with Type 2 Diabetes Risk in the North Indian Population

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Journal of Clinical and Biomedical Sciences

DOI: 10.58739/jcbs/v15i1.122

Year: 2025, Volume: 15, Issue: 1, Pages: 28-37

Original Article

Association of Intelectin Gene Polymorphism with Type 2 Diabetes Risk in the North Indian Population

Singh Shivangi¹, Chaturvedi Shubhangi¹, Pooja ¹, Modi Dinesh Raj^2∗, Singh Shefali³, Saxena Madhukar⁴, Katiyar Tridiv⁵

¹Research Scholar, Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, Uttar Pradesh, India
²Professor, Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, Uttar Pradesh, India
³Physician, Dr. OP Chaudhary Hospital and Research Centre, Lucknow, Uttar Pradesh, India
⁴Vice President operation, Chemzin Biotox Research Institute Pvt. Ltd, Baddi, Himachal Pradesh, India
⁵Research Associate, Department of Molecular Medicine & Biotechnology, SGPGIMS, Lucknow, Uttar Pradesh, India

*Corresponding Author
Email: [email protected]

Received Date:30 August 2024, Accepted Date:13 January 2025, Published Date:25 March 2025

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background: Intelectin 1 is an anti-inflammatory adipocytokine involved in the regulation of insulin secretion. Genetic variations in the coding region of Intelectin can cause hyperglycemia due to impaired glucose levels, increasing the susceptibility to Type 2 Diabetes Mellitus (T2DM). Objective: This study examines the genetic variations in the Intelectin 1 coding region, specifically rs2274907 and rs2274908. Materials and Methods: A case-control study was conducted with 300 participants: 150 individuals with Type 2 Diabetes Mellitus (fasting glucose >125 mg/dL) and 150 healthy controls (fasting glucose <125 mg/dL). The frequencies of rs2274907 and rs2274908 polymorphisms were analyzed using the Tetra Amplification refractory Mutation system and allele-specific PCR, respectively. Results: The study identified a significant association between specific gene variations and increased risk of Type 2 Diabetes mellitus. The TT genotype of rs2274907 showed a higher frequency in the diabetes group (15.33%) compared to the control group (3.33%), with a model of TT vs. AT+AA: X²=12.76, P=0.0004, OR=5.25, and 95% CI=1.93-14.21. Similarly, the AA genotype of rs2274908 was more frequent in the diabetes group (26.66%) compared to the control group (7.33%), with a model of AA vs. AG+GG: X²=18.52, P=0.0001, OR=4.59, and 95% CI=2.25-9.3. Conclusion: This is the first study of the North Indian population suggesting that specific Intelectin 1 gene variations (TT genotype of rs2274907 and AA genotype of rs2274908) are associated with an increased risk of Type 2 Diabetes Mellitus. Variations in Intelectin 1 may impact protein function and stability, serving as potential biomarkers for Type 2 Diabetes Mellitus and targets for personalized medicine.

Keywords: ITLN1, Case-control, Genetic variation, Type 2 Diabetes Mellitus

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