Journal of Clinical and Biomedical Sciences
Year: 2013, Volume: 3, Issue: 3, Pages: 146-149
Short communications/brief reports
Suresh T N*1, Harendra Kumar M L1, Thomas A K1 , Ramachandra Bhat1, Azeem M2, Myna2
1. Department of Pathology, Sri Devaraj Urs Medical College, Kolar, Karnataka. India.
2. Department of Otolaryngology and Head & Neck, Sri Devaraj Urs Medical College, Kolar, Karnataka. India.
*Corresponding Author
E mail : [email protected]
Background: Study of Sentinel Lymph Node (SNL) in clinically N0/ N1 stage helps to select the patients for neck dissection. Objective of this study is Pathological evaluation of Sentinel lymph node in oral squamous cell carcinoma. Materials and Methods: Retrospective study of Sentinel lymph node in 28 cases of oral squamous cell carcinoma with NO (6 cases) and N1 (22 cases) was undertaken. SLN were studied using imprint cytology (18 cases), haematoxylin and Eosin (H&E) staining (28 cases), serial step sectioning (SSS) at the interval of 50 microns with H&E staining and Immunohistochemistry using pancytokeratin AE1/AE2 antibody. Results: on detail pathological study SLN showed metastasis in 11 out of 28 cases. On histopathological correlation imprint cytology showed metastasis in 3 cases but in 2 cases there is false negativity. On histopathological correlation sensitivity, specificity and negative predictive value of imprint cytology is 60%, 100% and 87% respectively. Initial H&E section showed macrometastasis in 7 cases. Additionally SSS and immunohistochemistry demonstrated isolated tumour cells in 4 cases. Conclusion: Imprint cytology is less effective in identifying metastasis. Histopathology & Immunohistochemistry are required to identify micrometastasis & isolated tumour cells.
Key words: Imprint cytology, Metastasis, Oral cancer, Sentinel lymph node.
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